Feline Leukemia Virus (FeLV) RT-PCR testing provides highly sensitive molecular detection of this significant oncogenic retrovirus in cats. This advanced diagnostic method can detect viral RNA and proviral DNA in infected cells, enabling diagnosis at various stages of infection including early and regressive infections when antigen tests may be negative or ambiguous.
Proviral DNA Detection
Early Infection Identification
Regressive Infection Assessment
Viral Load Quantification
Maternal-Origin vs. Active Infection Distinction
Feline Leukemia Virus (FeLV) is an oncogenic retrovirus that incorporates its genetic material into the host's DNA, causing a variety of diseases including lymphoma, leukemia, immunosuppression, and bone marrow disorders. Infection outcomes include progressive (persistent viremia), regressive (transient viremia followed by immune control), and focal/atypical infections. RT-PCR testing can detect proviral DNA that remains integrated in host cells even after viremia has resolved, providing diagnostic clarity in complex cases where antigen testing may produce ambiguous results.
Clinical Significance of FeLV RT-PCR Testing
Infection Staging: Differentiate between progressive, regressive, and focal infections.
Oncogenic Risk: Assess potential for lymphoma and leukemia development.
Discordant Results: Resolve ambiguous or discordant results from antigen testing.
Transmission Prevention: Guide management decisions for multi-cat households.
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